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Literature summary for 3.1.13.4 extracted from

  • Kim, J.; Sambalkhundev, G.O.; Kim, S.; Son, J.; Han, A.R.; Ko, S.M.; Hwang, K.Y.; Lee, W.C.
    Processing of A-form ssDNA by cryptic RNase H fold exonuclease PF2046. (2016), Arch. Biochem. Biophys., 606, 143-150 .
    View publication on PubMed
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
gene PF2046 Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), X-ray diffraction structure determination and analysis Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate ?
-
 ?
additional information Pyrococcus furiosus ATCC 43587 RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate ?
-
 ?
additional information Pyrococcus furiosus Vc1 RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate ?
-
 ?
additional information Pyrococcus furiosus JCM 8422 RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZE9
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-
Pyrococcus furiosus ATCC 43587 Q8TZE9
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-
Pyrococcus furiosus JCM 8422 Q8TZE9
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-
Pyrococcus furiosus Vc1 Q8TZE9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate Pyrococcus furiosus ?
-
 ?
additional information RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate Pyrococcus furiosus ATCC 43587 ?
-
 ?
additional information RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate Pyrococcus furiosus Vc1 ?
-
 ?
additional information RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate Pyrococcus furiosus JCM 8422 ?
-
 ?

Synonyms

Synonyms Comment Organism
PF2046
-
 Pyrococcus furiosus
RNase H
-
 Pyrococcus furiosus

General Information

General Information Comment Organism
additional information in the structure of PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), PF2046 accommodates dT4 tightly in a groove and imposes steric hindrance on dT4 mainly by residue Phe220 such that dT4 assumes the A-form. As poly-dA prefer B-form due to the stereochemical restrictions, the A-form ssDNA binding by PF2046 disfavors the processing of poly-dA. Phe220 variants display reduced activity toward poly-dA and the A-form appears to be a prerequisite for the processing by PF2046 Pyrococcus furiosus