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Literature summary for 3.1.13.4 extracted from

  • Romano, M.; Van De Weerd, R.; Brouwer, F.; Roviello, G.; Lacroix, R.; Sparrius, M.; Van Den Brink-Van Stempvoort, G.; Maaskant, J.; Van Der Sar, A.; Appelmelk, B.; Geurtsen, J.; Berisio, R.
    Structure and function of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo (2014), Structure, 22, 719-730.
    View publication on PubMed
Search for more literature for 3.1.13.4

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.1 A resolution, one dimer per asymmetric unit Mycobacterium marinum

Protein Variants

Protein Variants Comment Organism
H140A complete loss of activity Mycobacterium marinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18500
-
 2 * 18500, calculated Mycobacterium marinum
38460
-
 gel filtration coupled with multiangle light scattering Mycobacterium marinum

Organism

Organism UniProt Comment Textmining
Mycobacterium marinum B2HGU6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(A)12 + H2O
-
 Mycobacterium marinum AMP full degradation of substrate ?
AAAACAAAAA + H2O
-
 Mycobacterium marinum AMP + ? plus a further degradation product containing four A nucleotides and one C nucleotide ?
additional information enzyme shows strong specificity toward polyadenylate nucleotides, no degradation of poly(C), poly(G), and poly(U) substrates Mycobacterium marinum ?
-
 ?
poly(A) + H2O
-
 Mycobacterium marinum AMP + ? no accumulation of polynucleotides ?

Subunits

Subunits Comment Organism
dimer 2 * 18500, calculated Mycobacterium marinum

Synonyms

Synonyms Comment Organism
MMAR_3223
-
 Mycobacterium marinum
RNase AS
-
 Mycobacterium marinum

General Information

General Information Comment Organism
physiological function infection of zebra fish with a RNase AS deletion mutant strain is reduced to 4.7% of wild-type. The knockout can be complemented by Mycobacterium tuberculosis gene rv2179c encoding a 3'-5' exoribonuclease Mycobacterium marinum