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Literature summary for 3.1.13.4 extracted from

  • Balatsos, N.A.; Anastasakis, D.; Stathopoulos, C.
    Inhibition of human poly(A)-specific ribonuclease (PARN) by purine nucleotides: kinetic analysis (2009), J. Enzyme Inhib. Med. Chem., 24, 516-523.
    View publication on PubMed
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed as N-terminal His6-tagged polypeptide in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP purine monophosphate (RMP) and diphosphate nucleotides (RDP) exhibit competitive inhibition, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
AMP purine monophosphate (RMP) and diphosphate nucleotides (RDP) exhibit competitive inhibition, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
ATP purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
deoxyATP purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
deoxyGTP purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
GDP purine monophosphate (RMP) and diphosphate nucleotides (RDP) exhibit competitive inhibition, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
GMP purine monophosphate (RMP) and diphosphate nucleotides (RDP) exhibit competitive inhibition, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
GTP purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens
purine nucleotides purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors while purine monophosphate (RMP) and diphosphate nucleotides (RDP) exhibit competitive inhibition, furthermore PARN does not discriminate whether there is ribose or deoxy-ribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + H2O
-
 Homo sapiens AMP
-
 ir

Synonyms

Synonyms Comment Organism
PARN
-
 Homo sapiens
poly(A)-specific ribonuclease
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 ADP at pH 7.0, 30°C Homo sapiens
1.6
-
 AMP at pH 7.0, 30°C Homo sapiens
2
-
 deoxyGTP at pH 7.0, 30°C Homo sapiens
2.8
-
 GDP at pH 7.0, 30°C Homo sapiens
2.8
-
 deoxyATP at pH 7.0, 30°C Homo sapiens
3.2
-
 ATP at pH 7.0, 30°C Homo sapiens
3.3
-
 GTP at pH 7.0, 30°C Homo sapiens
4.5
-
 GMP at pH 7.0, 30°C Homo sapiens