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Literature summary for 3.1.13.4 extracted from

  • Liu, W.F.; Zhang, A.; Cheng, Y.; Zhou, H.M.; Yan, Y.B.
    Allosteric regulation of human poly(A)-specific ribonuclease by cap and potassium ions (2009), Biochem. Biophys. Res. Commun., 379, 341-345.
    View publication on PubMed
Search for more literature for 3.1.13.4

Activating Compound

Activating Compound Comment Organism Structure
K+ allosteric activator of PARN and its truncated forms, optimal concentration is around 100 mM, with the increase of the K+ concentration, the enzyme reaches its Vmax at a much lower substrate concentration Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of full-length PARN and truncated forms p62 and p54 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cap analogue 7-methylguanosine(5')ppp(5')G allosteric inhibitor of PARN in the presence of a physiological K+ concentration Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the binding of K+ to the entire RNA-recognition motif domain, which plays a central role in the allosteric communications of PARN regulation, leads to an increase of substrate-binding affinity but a decrease in the cooperativity of the substrate-binding site, while the binding of the cap analogue decreases both the catalytic efficiency and the substrate-binding affinity Homo sapiens
Mg2+ essential for PARN activity and stability Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 PARN (p54), residues 1-470 Homo sapiens
62000
-
 PARN (p62), residues 1-540 Homo sapiens
74000
-
 full-length PARN (p74) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PARN specifically degrades the mRNA poly(A) tails with a free 3'-hydroxyl group and releases 5'-AMP as the mononucleotide product ?
-
 ?
poly(A) RNA + H2O Homo sapiens deadenylation of mRNA 5'-AMP + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of full-length PARN and truncated forms p62 and p54 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Vmax value is 0.30 micromol/min/micromol for the full-length enzyme at 25 mM K+ Homo sapiens
additional information
-
 Vmax value is 0.36 micromol/min/micromol for the full-length enzyme at 50 mM K+ Homo sapiens
additional information
-
 Vmax value is 0.42 micromol/min/micromol for the full-length enzyme at 100 mM K+ Homo sapiens
additional information
-
 Vmax value is 0.42 micromol/min/micromol for the full-length enzyme at 200 mM K+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PARN specifically degrades the mRNA poly(A) tails with a free 3'-hydroxyl group and releases 5'-AMP as the mononucleotide product Homo sapiens ?
-
 ?
poly(A) RNA + H2O deadenylation of mRNA Homo sapiens 5'-AMP + ?
-
 ?

Subunits

Subunits Comment Organism
dimer multi-domain enzyme Homo sapiens

Synonyms

Synonyms Comment Organism
PARN
-
 Homo sapiens
poly(A)-specific ribonuclease
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens