Application | Comment | Organism |
---|---|---|
additional information | Mg2+ has dual effects on PARN stability, protecting the active site against denaturation, but destabilizing the overall structural stability of the protein | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
plasmid pET33 containing the human parn gene expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 3 mM gradually decreases activity about 15fold | Homo sapiens | |
Co2+ | 3 mM gradually decreases activity about 78fold | Homo sapiens | |
Mg2+ | behaves as a destabilizer of the overall structural stability of PARN, promotes thermal unfolding and aggregation at high temperatures | Homo sapiens | |
Mn2+ | 3 mM gradually decreases activity about 5fold | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can protect PARN against thermal inactivation by increasing the midpoint of inactivation and decreasing the inactivation rate, protective effect is unique to Mg2+ in a concentration-dependent manner | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni2+ affinity chromatography and gel filtration, purity above 98% | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) RNA + H2O | - |
Homo sapiens | 5'-AMP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
in the absence of Mg2+, the activity of PARN decreases continuously with increasing temperature | Homo sapiens |