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Literature summary for 3.1.13.4 extracted from

  • Zhang, A.; Liu, W.F.; Yan, Y.B.
    Role of the RRM domain in the activity, structure and stability of poly(A)-specific ribonuclease (2007), Arch. Biochem. Biophys., 461, 255-262.
    View publication on PubMed
Search for more literature for 3.1.13.4

Application

Application Comment Organism
additional information the entire RRM domain not only contributes to the substrate binding and efficient catalysis of PARN, but also stabilizes the overall structures of the protein. p46 contains only one Trp residue (W219), p54 contains two (W219 and W456), and p74 contains six (W219, W456, W474, W526, W531 and W639) Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET28a, expressed in Escherichia coli BL21(DE3)pLys(S) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride full-length enzyme retains most of its activity at guanidine hydrochloride concentrations below 0.5 M, whereas an abrupt decrease of the activity of p54 and p46 is found when guanidine hydrochloride concentration is increased Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000236
-
 poly(A) RNA full-length 74 kDa PARN Homo sapiens
0.0000303
-
 poly(A) RNA 54 kDa form Homo sapiens
0.0000333
-
 poly(A) RNA 46 kDa form Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 truncated form completely missing the RRM Homo sapiens
54000
-
 proteolytic fragment with half of the RRM Homo sapiens
74000
-
 full-length PARN Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA resin and by gel filtration, purity above 98% Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) RNA + H2O
-
 Homo sapiens 5'-AMP + ?
-
 ?

Synonyms

Synonyms Comment Organism
PARN
-
 Homo sapiens