Application | Comment | Organism |
---|---|---|
additional information | the entire RRM domain not only contributes to the substrate binding and efficient catalysis of PARN, but also stabilizes the overall structures of the protein. p46 contains only one Trp residue (W219), p54 contains two (W219 and W456), and p74 contains six (W219, W456, W474, W526, W531 and W639) | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into vector pET28a, expressed in Escherichia coli BL21(DE3)pLys(S) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | full-length enzyme retains most of its activity at guanidine hydrochloride concentrations below 0.5 M, whereas an abrupt decrease of the activity of p54 and p46 is found when guanidine hydrochloride concentration is increased | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000236 | - |
poly(A) RNA | full-length 74 kDa PARN | Homo sapiens | |
0.0000303 | - |
poly(A) RNA | 54 kDa form | Homo sapiens | |
0.0000333 | - |
poly(A) RNA | 46 kDa form | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
truncated form completely missing the RRM | Homo sapiens |
54000 | - |
proteolytic fragment with half of the RRM | Homo sapiens |
74000 | - |
full-length PARN | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA resin and by gel filtration, purity above 98% | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) RNA + H2O | - |
Homo sapiens | 5'-AMP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Homo sapiens |