Cloned (Comment) | Organism |
---|---|
expression of enzyme subunits in the two-hybrid system using strain L40, expression of lexA-tagged wild-type and mutant Pab1p, Pan2p, and Pan3p in Escherichia coli, expression of full-length and N-terminal constructs of factor Pbp1p in Escherichia coli, overview | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A519V | site-directed mutagenesis of Pab1p, the mutant shows no resistance to 3-AT in contrast to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
G444D | site-directed mutagenesis of Pab1p, the mutant shows no resistance to 3-AT in contrast to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
G528D | site-directed mutagenesis of Pab1p, the mutant shows no resistance to 3-AT in contrast to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
additional information | growth of cells transformed with mutant constructs, overview | Saccharomyces cerevisiae |
R506G | site-directed mutagenesis of Pab1p, the mutant shows no resistance to 3-AT in contrast to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
V451A | site-directed mutagenesis of Pab1p, the mutant shows no resistance to 3-AT in contrast to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
Y514C | site-directed mutagenesis of Pab1p, the mutant shows reduced resistance to 3-AT compared to the wild-type protein, identification of interaction residues and domains with Ppb1p, Pan2p and Pan3p | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-aminotriazole | competitive inhibitor | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the enzyme plays an important role in the posttranscriptional maturation of mRNA poly(A) tails, factors involved in positive, e.g. Pan3p, and negative, e.g. Pbp1p, regulation, schematic model, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains L40 and yAS1255, a pab1DELTA strain, and a pan3DELTA strain yME43 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme plays an important role in the posttranscriptional maturation of mRNA poly(A) tails, factors involved in positive, e.g. Pan3p, and negative, e.g. Pbp1p, regulation, schematic model, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | protein-protein interaction specificity, Pab1p interacts with Pan3p and Pbp1p, which also interact with each other, Pbp1p interacts with itself, binding pocket structures, overview | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme requires the subunits Pan2p and Pan3p, as well as the poly(A)-binding protein Pab1p for activity | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PAN | - |
Saccharomyces cerevisiae |
poly(A) nuclease | - |
Saccharomyces cerevisiae |