Literature summary for 3.1.13.4 extracted from

Ren, Y.G.; Martinez, J.; Virtanen, A.
Identification of the active site of poly(A)-specific ribonuclease by site-directed mutagenesis and Fe(2+)-mediated cleavage (2002), J. Biol. Chem., 277, 5982-5987.

Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D28A	catalytically inactive, RNA-binding is not affected	Homo sapiens
D292A	catalytically inactive, RNA-binding is not affected	Homo sapiens
D382A	catalytically inactive, RNA-binding is not affected	Homo sapiens
E30A	catalytically inactive, RNA-binding is not affected	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	can substitute for Mg2+, Mn2+, optimal at 0.01 mM, two binding sites	Homo sapiens
Mg2+	or Mn2+, required	Homo sapiens
Mn2+	or Mg2+, required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzymes	Homo sapiens

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Release 2023.1 (January 2023)