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Literature summary for 3.1.13.2 extracted from

  • Nguyen, T.N.; Angkawidjaja, C.; Kanaya, E.; Koga, Y.; Takano, K.; Kanaya, S.
    Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1 (2012), Protein Sci., 21, 553-561.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MIC2067(DE3) cells Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 0.2 M NaCl, 0.4 M NaH2PO4, and 1.6 M K2HPO4 Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activity with Ca2+ Sulfurisphaera tokodaii
Na+ LC11-RNase H1 exhibits the highest activity in the presence of 10 mM NaCl. Its activity gradually decreases as the salt concentration increases Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15600
-
 1 * 15600, LC11-RNase H1, calculated from amino acid sequence Sulfurisphaera tokodaii
16000
-
 1 * 16000, LC11-RNase H1, SDS-PAGE Sulfurisphaera tokodaii
18000
-
 LC11-RNase H1, gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Sulfurisphaera tokodaii
-
 ?
-
 ?
DNA-RNA hybrid + H2O Sulfurisphaera tokodaii DSM 16993
-
 ?
-
 ?
double-stranded RNA + H2O Sulfurisphaera tokodaii substrate of RNase H1 ?
-
 ?
double-stranded RNA + H2O Sulfurisphaera tokodaii DSM 16993 substrate of RNase H1 ?
-
 ?
additional information Sulfurisphaera tokodaii LC11-RNase H1 does not exhibit double strand RNase activity ?
-
 ?
additional information Sulfurisphaera tokodaii DSM 16993 LC11-RNase H1 does not exhibit double strand RNase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii F9VN79
-
-
Sulfurisphaera tokodaii DSM 16993 F9VN79
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography and Superdex 200 gel filtration Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
DNA-RNA hybrid + H2O
-
 Sulfurisphaera tokodaii DSM 16993 ?
-
 ?
double-stranded RNA + H2O substrate of RNase H1 Sulfurisphaera tokodaii ?
-
 ?
double-stranded RNA + H2O substrate of RNase H1 Sulfurisphaera tokodaii DSM 16993 ?
-
 ?
additional information LC11-RNase H1 does not exhibit double strand RNase activity Sulfurisphaera tokodaii ?
-
 ?
additional information LC11-RNase H1 does not exhibit double strand RNase activity Sulfurisphaera tokodaii DSM 16993 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 15600, LC11-RNase H1, calculated from amino acid sequence Sulfurisphaera tokodaii
monomer 1 * 16000, LC11-RNase H1, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
LC11-RNase H1 metagenome-derived LC11-RNase H1 is a homolog of Sulfolobus tokodaii RNase H1 that lacks a C-terminal tail, which is responsible for hyperstabilization Sulfurisphaera tokodaii
RNase H1
-
 Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 optimum pH for LC11-RNase H1 Sulfurisphaera tokodaii