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Literature summary for 3.1.13.2 extracted from

  • Tannous, E.; Yokoyama, K.; You, D.J.; Koga, Y.; Kanaya, S.
    A dual role of divalent metal ions in catalysis and folding of RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1 (2012), FEBS Open Bio, 2, 345-352.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3) cells Halobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the optimum concentration of Mg2+ for activity in the presence of 3 M NaCl is 20 mM Halobacterium sp.
Mn2+ the optimum concentration of Mn2+ for activity in the presence of 3 M NaCl is 1 mM Halobacterium sp.
additional information the enzyme requires either salt or divalent metal ions for folding and is incompletely folded in the absence of both of them Halobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12 bp RNA/DNA hybrid + H2O Halobacterium sp. 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA ?
-
?
12 bp RNA/DNA hybrid + H2O Halobacterium sp. NRC-1 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA ?
-
?

Organism

Organism UniProt Comment Textmining
Halobacterium sp.
-
-
-
Halobacterium sp. NRC-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography, Mono Q column chromatography, and Superdex 200pg gel filtration Halobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12 bp RNA/DNA hybrid + H2O 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA Halobacterium sp. ?
-
?
12 bp RNA/DNA hybrid + H2O 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA Halobacterium sp. NRC-1 ?
-
?

Synonyms

Synonyms Comment Organism
RNase H1
-
Halobacterium sp.
RNH1
-
Halobacterium sp.