Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3) cells | Halobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the optimum concentration of Mg2+ for activity in the presence of 3 M NaCl is 20 mM | Halobacterium sp. | |
Mn2+ | the optimum concentration of Mn2+ for activity in the presence of 3 M NaCl is 1 mM | Halobacterium sp. | |
additional information | the enzyme requires either salt or divalent metal ions for folding and is incompletely folded in the absence of both of them | Halobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
12 bp RNA/DNA hybrid + H2O | Halobacterium sp. | 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA | ? | - |
? | |
12 bp RNA/DNA hybrid + H2O | Halobacterium sp. NRC-1 | 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium sp. | - |
- |
- |
Halobacterium sp. NRC-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, Mono Q column chromatography, and Superdex 200pg gel filtration | Halobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12 bp RNA/DNA hybrid + H2O | 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA | Halobacterium sp. | ? | - |
? | |
12 bp RNA/DNA hybrid + H2O | 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA | Halobacterium sp. NRC-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H1 | - |
Halobacterium sp. |
RNH1 | - |
Halobacterium sp. |