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Literature summary for 3.1.13.2 extracted from

  • Alla, N.R.; Nicholson, A.W.
    Evidence for a dual functional role of a conserved histidine in RNA-DNA heteroduplex cleavage by human RNase H1 (2012), FEBS J., 279, 4492-4500.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)[pLysS] cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H264A the mutation causes an about 100fold decrease in kcat under multiple-turnover conditions, but does not alter the Km Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000097
-
DNA-RNA hybrid wild type enzyme, at pH 7.5 and 30°C Homo sapiens
0.000106
-
DNA-RNA hybrid mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM Co2+ supports catalytic activity Homo sapiens
K+ optimal concentration of 100-150 mM Homo sapiens
Mg2+ preferred cofactor, optimal concentration of 10 mM Homo sapiens
Mn2+ 5 mM Mn2+ supports catalytic activity Homo sapiens
additional information not influenced by Ca2+, Ni2+ and Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap HP Ni-NTA column chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RNase H1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
DNA-RNA hybrid mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens
3.5
-
DNA-RNA hybrid wild type enzyme, at pH 7.5 and 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
240
-
DNA-RNA hybrid mutant enzyme H264A, at pH 7.5 and 30°C Homo sapiens
36000
-
DNA-RNA hybrid wild type enzyme, at pH 7.5 and 30°C Homo sapiens