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Literature summary for 3.1.13.2 extracted from
- Structural and inhibition studies of the RNase H function of xenotropic murine leukemia virus-related virus reverse transcriptase (2012), Antimicrob. Agents Chemother., 56, 2048-2061.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Moloney murine leukemia virus |
| expressed in Escherichia coli | Human immunodeficiency virus 1 |
| expressed in Escherichia coli | Xenotropic MuLV-related virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7 | Xenotropic MuLV-related virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Human immunodeficiency virus 1 |  |
| 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Moloney murine leukemia virus | |
| 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | potent inhibitor | Xenotropic MuLV-related virus | |
| 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Human immunodeficiency virus 1 | |
| 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Moloney murine leukemia virus | |
| 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | - |
Xenotropic MuLV-related virus | |
| beta-thujaplicinol | - |
Human immunodeficiency virus 1 | |
| beta-thujaplicinol | - |
Moloney murine leukemia virus | |
| beta-thujaplicinol | able to inhibit RNase H in the presence of 200 nM HTS-1 RNA-DNA substrate. However, beta-thujaplicinol is not able to inhibit RNase H as the concentration of RNA-DNA substrate is increased | Xenotropic MuLV-related virus | |
| trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Human immunodeficiency virus 1 | |
| trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Moloney murine leukemia virus | |
| trihydroxybenzoylbiphenyl carboxylate hydrazone | - |
Xenotropic MuLV-related virus | |
| trihydroxybenzoylnaphthyl hydrazone | - |
Human immunodeficiency virus 1 | |
| trihydroxybenzoylnaphthyl hydrazone | - |
Moloney murine leukemia virus | |
| trihydroxybenzoylnaphthyl hydrazone | - |
Xenotropic MuLV-related virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | RNase H activity is slightly faster in the presence of Mg2+ than Mn2+ | Human immunodeficiency virus 1 | |
| Mn2+ | optimal RNase H activitiy in the presence of Mn2+ and not Mg2+ | Moloney murine leukemia virus | |
| Mn2+ | optimal RNase H activitiy in the presence of Mn2+ and not Mg2+ | Xenotropic MuLV-related virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | - |
- |
- |
| Moloney murine leukemia virus | - |
- |
- |
| Xenotropic MuLV-related virus | A1Z651 | clone VP62 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GST column chromatography and gel filtration | Moloney murine leukemia virus |
| GST column chromatography and gel filtration | Human immunodeficiency virus 1 |
| GST column chromatography and gel filtration | Xenotropic MuLV-related virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 18 base pair upstream from the 3' primer end | Human immunodeficiency virus 1 | ? | - |
? | |
| DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 19 base pair upstream from the 3' primer end | Moloney murine leukemia virus | ? | - |
? | |
| DNA-RNA hybrid + H2O | a Cy3-Tr35/Pd22 RNA-DNA hybrid is cut at approximately 19 base pair upstream from the 3' primer end | Xenotropic MuLV-related virus | ? | - |
? | |
| HTS-1 RNA-DNA + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
| HTS-1 RNA-DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
| HTS-1 RNA-DNA + H2O | - |
Xenotropic MuLV-related virus | ? | - |
? | |
| HTS-2 RNA-DNA + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
| HTS-2 RNA-DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
| HTS-2 RNA-DNA + H2O | - |
Xenotropic MuLV-related virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H | - |
Moloney murine leukemia virus |
| RNase H | - |
Human immunodeficiency virus 1 |
| RNase H | - |
Xenotropic MuLV-related virus |
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