Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.2 extracted from

  • Wisniewski, M.; Balakrishnan, M.; Palaniappan, C.; Fay, P.J.; Bambara, R.A.
    Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H (2000), Proc. Natl. Acad. Sci. USA, 97, 11978-11983.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
HIV-1
-

Reaction

Reaction Comment Organism Reaction ID
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid The enzyme reverse transcriptase has polymerase and RNase H activities, study of progressive cleavage mechanism of enzyme. Because the residual RNAs vary in length, longer RNAs require several additional cleavage events for complete removal. Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3' end-labeled 41-nt RNA annealed to a 77-nt DNA template
-
Human immunodeficiency virus 1 DNA + RNA fragments the most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts ?
5' end-labeled 41-nt RNA annealed to a 77-nt DNA template
-
Human immunodeficiency virus 1 DNA + RNA fragments The first product observed is the primary cut. The primary cut occurs faster than the secondary cut ?
5' end-labeled 50-nt RNA annealed to a 77-nt DNA template
-
Human immunodeficiency virus 1 DNA + RNA fragments The first product observed is the primary cut. The primary cut occurs faster than the secondary cut ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information study of the order of cleavage, most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts within 1 min Human immunodeficiency virus 1