Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
HIV-1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid | The enzyme reverse transcriptase has polymerase and RNase H activities, study of progressive cleavage mechanism of enzyme. Because the residual RNAs vary in length, longer RNAs require several additional cleavage events for complete removal. | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3' end-labeled 41-nt RNA annealed to a 77-nt DNA template | - |
Human immunodeficiency virus 1 | DNA + RNA fragments | the most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts | ? | |
5' end-labeled 41-nt RNA annealed to a 77-nt DNA template | - |
Human immunodeficiency virus 1 | DNA + RNA fragments | The first product observed is the primary cut. The primary cut occurs faster than the secondary cut | ? | |
5' end-labeled 50-nt RNA annealed to a 77-nt DNA template | - |
Human immunodeficiency virus 1 | DNA + RNA fragments | The first product observed is the primary cut. The primary cut occurs faster than the secondary cut | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | study of the order of cleavage, most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts within 1 min | Human immunodeficiency virus 1 |