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Literature summary for 3.1.13.2 extracted from

  • Lener, D.; Budihas, S.R.; Le Grice, S.F.J.
    Mutating conserved residues in the ribonuclease H domain of Ty3 reverse transcriptase affects specialized cleavage events (2002), J. Biol. Chem., 277, 26486-26495.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D358N complete loss of Mg2+ and Mn2+-dependent RNase H activity Human immunodeficiency virus 1
D426N complete loss of Mg2+ and Mn2+-dependent RNase H activity Human immunodeficiency virus 1
D469N enhanced Mn2+ dependent RNase H activity in comparison to Mg2+ dependent RNase H activity Human immunodeficiency virus 1
E401Q complete loss of Mg2+ and Mn2+-dependent RNase H activity Human immunodeficiency virus 1
H427A enhanced Mn2+ dependent RNase H activity in comparison to Mg2+ dependent RNase H activity Human immunodeficiency virus 1
Y459A enhanced Mn2+ dependent RNase H activity in comparison to Mg2+ dependent RNase H activity Human immunodeficiency virus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Asp358, Glu401 and Asp426 constitute the primary Mg2+ binding site of Ty3 RNase H domain Human immunodeficiency virus 1
Mn2+
-
 Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1 P03366 HIV-1
-

Reaction

Reaction Comment Organism Reaction ID
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid the enzyme reverse transcriptase has polymerase and RNase H activities Human immunodeficiency virus 1
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid duplex + H2O
-
 Human immunodeficiency virus 1 oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?

Synonyms

Synonyms Comment Organism
RT/RNase H
-
 Human immunodeficiency virus 1