Cloned (Comment) | Organism |
---|---|
- |
Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
Y501A | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501E | 25% activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501F | 85% activity in comparison to wild-type enzyme, the mutant remains sensitive to N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone inhibition | Human immunodeficiency virus 1 |
Y501G | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501H | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501L | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501Q | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501R | 90% activity in comparison to wild-type enzyme, the mutant is completely resistant to N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone inhibition | Human immunodeficiency virus 1 |
Y501S | complete loss of activity in comparison to wild-type enzyme | Human immunodeficiency virus 1 |
Y501W | 100% activity in comparison to wild-type enzyme, 6fold decreased sensitivity to inhibition N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone | BBNH, the residue Tyr501 is integral in the binding interaction, mechanism | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | P03366 | HIV-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of relative activities of wild-type and mutant enzymes | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5' RNA-DNA duplex + H2O | - |
Human immunodeficiency virus 1 | oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RT RNase H | - |
Human immunodeficiency virus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone | pH 7.9, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0047 | - |
N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone | pH 7.9, 37°C, mutant Y501F | Human immunodeficiency virus 1 | |
0.0206 | - |
N-(4-tert-butylbenzoyl)-2-hydroxynaphthaldehyde hydrazone | pH 7.9, 37°C, mutant Y501W | Human immunodeficiency virus 1 |