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Literature summary for 3.1.13.2 extracted from

  • Wei, X.; Peterson, D.L.
    Expression, purification and characterization of an active RNase H domain of the Hepatitis B viral polymerase (1996), J. Biol. Chem., 271, 32617-32622.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
KCl maximal enzyme activity at 0-20 mM Hepatitis B virus

Cloned(Commentary)

Cloned (Comment) Organism
RNase H domain Hepatitis B virus

Inhibitors

Inhibitors Comment Organism Structure
KCl at concentrations greater than 20 mM Hepatitis B virus
Mg2+ inhibitory at concentrations greater than 10 mM Hepatitis B virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity at 2mM Hepatitis B virus
Mg2+ preference for Mg2+ to Mn2+ Hepatitis B virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
Ni-Sepharose column chromatography, amino acid calculation Hepatitis B virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid duplex + H2O Hepatitis B virus
-
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?
DNA-RNA hybrid duplex + H2O Hepatitis B virus HBV
-
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?

Organism

Organism UniProt Comment Textmining
Hepatitis B virus
-
HBV
-
Hepatitis B virus HBV
-
HBV
-
More
-
retrovirus: RNaseH and transcriptase are present in a single polypeptide
-

Oxidation Stability

Oxidation Stability Organism
easily inactivated by oxidation, no activity detected in absence of dithiothreitol or mercaptoethanol Hepatitis B virus

Purification (Commentary)

Purification (Comment) Organism
RNase H domain Hepatitis B virus

Renatured (Commentary)

Renatured (Comment) Organism
-
Hepatitis B virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid duplex + H2O
-
Hepatitis B virus oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?
DNA-RNA hybrid duplex + H2O poor substrate: poly(rG)-poly(dC) Hepatitis B virus oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?
DNA-RNA hybrid duplex + H2O
-
Hepatitis B virus HBV oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?
DNA-RNA hybrid duplex + H2O poor substrate: poly(rG)-poly(dC) Hepatitis B virus HBV oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
for 10 min, does not affect the reaction Hepatitis B virus
100
-
for 10 min, no activity remains Hepatitis B virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Hepatitis B virus