Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal enzyme activity at 0-20 mM | Hepatitis B virus |
Cloned (Comment) | Organism |
---|---|
RNase H domain | Hepatitis B virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | at concentrations greater than 20 mM | Hepatitis B virus | |
Mg2+ | inhibitory at concentrations greater than 10 mM | Hepatitis B virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximum activity at 2mM | Hepatitis B virus | |
Mg2+ | preference for Mg2+ to Mn2+ | Hepatitis B virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
Ni-Sepharose column chromatography, amino acid calculation | Hepatitis B virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid duplex + H2O | Hepatitis B virus | - |
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? | |
DNA-RNA hybrid duplex + H2O | Hepatitis B virus HBV | - |
oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepatitis B virus | - |
HBV | - |
Hepatitis B virus HBV | - |
HBV | - |
More | - |
retrovirus: RNaseH and transcriptase are present in a single polypeptide | - |
Oxidation Stability | Organism |
---|---|
easily inactivated by oxidation, no activity detected in absence of dithiothreitol or mercaptoethanol | Hepatitis B virus |
Purification (Comment) | Organism |
---|---|
RNase H domain | Hepatitis B virus |
Renatured (Comment) | Organism |
---|---|
- |
Hepatitis B virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid duplex + H2O | - |
Hepatitis B virus | oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? | |
DNA-RNA hybrid duplex + H2O | poor substrate: poly(rG)-poly(dC) | Hepatitis B virus | oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? | |
DNA-RNA hybrid duplex + H2O | - |
Hepatitis B virus HBV | oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? | |
DNA-RNA hybrid duplex + H2O | poor substrate: poly(rG)-poly(dC) | Hepatitis B virus HBV | oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
for 10 min, does not affect the reaction | Hepatitis B virus |
100 | - |
for 10 min, no activity remains | Hepatitis B virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Hepatitis B virus |