Cloned (Comment) | Organism |
---|---|
Expression of rnr mutant (inactivation of Rnase R) is performed in Escherichia coli. Overexpression of genes corresponding to the flagellar apparatus or to the biosynthesis of cofactors by the absence of RNase R gene. | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | mRNA is a direct substrate for RNase R | ? | - |
? | |
additional information | Pseudomonas putida | RNase R is a 3'-5'-exoribonuclease that is very processive and can efficiently digest RNAs having extensive secondary structures, such as rRNA, RNAs containing repetitive extragenic palindromic (REP) sequences, or the transfer-messenger RNA required for trans-translation. | ? | - |
? | |
additional information | Pseudomonas putida | RNase R is also involved in the processing of 16S and 5S rRNA | ? | - |
? | |
additional information | Pseudomonas putida | The absence of RNase R leads to moderate increases in the mRNA levels of some RNases and RNA helicases, but other RNases and RNA helicases are not affected. | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | mRNA is a direct substrate for RNase R | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | RNase R is a 3'-5'-exoribonuclease that is very processive and can efficiently digest RNAs having extensive secondary structures, such as rRNA, RNAs containing repetitive extragenic palindromic (REP) sequences, or the transfer-messenger RNA required for trans-translation. | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | RNase R is also involved in the processing of 16S and 5S rRNA | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | The absence of RNase R leads to moderate increases in the mRNA levels of some RNases and RNA helicases, but other RNases and RNA helicases are not affected. | ? | - |
? | |
RNA + H2O | Pseudomonas putida | RNA turnover | phosphomononucleotides | - |
? | |
RNA + H2O | Pseudomonas putida KT 2240 | RNA turnover | phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88DE8 | - |
- |
Pseudomonas putida KT 2240 | Q88DE8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mRNA is a direct substrate for RNase R | Pseudomonas putida | ? | - |
? | |
additional information | RNase R is a 3'-5'-exoribonuclease that is very processive and can efficiently digest RNAs having extensive secondary structures, such as rRNA, RNAs containing repetitive extragenic palindromic (REP) sequences, or the transfer-messenger RNA required for trans-translation. | Pseudomonas putida | ? | - |
? | |
additional information | RNase R is also involved in the processing of 16S and 5S rRNA | Pseudomonas putida | ? | - |
? | |
additional information | The absence of RNase R leads to moderate increases in the mRNA levels of some RNases and RNA helicases, but other RNases and RNA helicases are not affected. | Pseudomonas putida | ? | - |
? | |
additional information | mRNA is a direct substrate for RNase R | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | RNase R is a 3'-5'-exoribonuclease that is very processive and can efficiently digest RNAs having extensive secondary structures, such as rRNA, RNAs containing repetitive extragenic palindromic (REP) sequences, or the transfer-messenger RNA required for trans-translation. | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | RNase R is also involved in the processing of 16S and 5S rRNA | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | The absence of RNase R leads to moderate increases in the mRNA levels of some RNases and RNA helicases, but other RNases and RNA helicases are not affected. | Pseudomonas putida KT 2240 | ? | - |
? | |
RNA + H2O | RNA turnover | Pseudomonas putida | phosphomononucleotides | - |
? | |
RNA + H2O | RNA turnover | Pseudomonas putida KT 2240 | phosphomononucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ribonuclease R | - |
Pseudomonas putida |
RNase R | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Following cell growth at 10°C by counting viable cells, rather than by turbidity, yields very similar growth rates. The doubling times are about 7 h for the wildtype strain and 15.5 h for the rnr mutant strain. | Pseudomonas putida |
additional information | - |
In LB medium, inactivation of the rnr gene impairs growth at 4°C, but not at 30°C (dispensability of RNase R). Other nucleases can perform its role under these conditions | Pseudomonas putida |
additional information | - |
Significant reduction of the growth rate at 10°C: the doubling time increased from 6 h in the wild-type strain to 16.5 h in the rnr mutant strain. | Pseudomonas putida |
additional information | - |
The expression of the rnr gene increases less than twofold after a 2-h cold shock in which the culture temperature is decreased from 30°C to 10°C. | Pseudomonas putida |