Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IPTG | 1 mM, after 2 h RNase major protein in cell extracts of E.coli strain BL21(DE3) | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
Escherichia coli strain JM109 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | metal binding | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | for catalysis of mRNA but not for substrate binding | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
and BL21(DE3) | - |
Escherichia coli JM109 | - |
and BL21(DE3) | - |
no activity in Escherichia coli | - |
carrying the mutant allele rnb296, single substitution of aspartate 209 for asparagine | - |
no activity in Escherichia coli SK4803 | - |
carrying the mutant allele rnb296, single substitution of aspartate 209 for asparagine | - |
Purification (Comment) | Organism |
---|---|
Escherichia coli strain BL21(DE3) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
m-RNA + H2O | SL9A, containing one stem-loop structure, and malE-malF operon, containing two stem-loop structures | Escherichia coli | ? | - |
? | |
m-RNA + H2O | SL9A, containing one stem-loop structure, and malE-malF operon, containing two stem-loop structures | Escherichia coli JM109 | ? | - |
? | |
poly[8-3H]adenylic acid + H2O | linear substrate, activity below 325 UE·micro g-1 | Escherichia coli | ? | - |
? | |
poly[8-3H]adenylic acid + H2O | linear substrate, activity below 325 UE·micro g-1 | Escherichia coli JM109 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease II | - |
Escherichia coli |
RNase II | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
EDTA | malE-malF mRNA transcripts incubated at 37°C | Escherichia coli |