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Literature summary for 3.1.13.1 extracted from

  • Caruccio, N.; Ross, J.
    Purification of a human polyribosome-associated 3'-5'exoribonuclease (1994), J. Biol. Chem., 269, 31814-31821.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by RNase A-type inhibitor Homo sapiens
poly(A) binding protein inhibits degradation of poly(A)+-strands Homo sapiens
RNA with a terminal 3'-phosphate group on the potential substrate molecule Homo sapiens
ss DNA oligonucleotides
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
polysome
-
Homo sapiens 5844
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ necessary for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
SDS-PAGE Homo sapiens
37000
-
native state gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Homo sapiens
-
5'-phosphomononucleotides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
5300-26500fold, to homogeneity Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythroleukemia cell
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, 10-ml fractions containing 1 mg trypsin inhibitor carrier protein because RNase II loses activity under this conditions at protein concentrations below 0.1 mg/ml Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex RNA + H2O
-
Homo sapiens ?
-
?
additional information 3'-phosphorylated RNA is not hydrolyzed Homo sapiens ?
-
?
mRNA + H2O
-
Homo sapiens 5'-phosphomononucleotides
-
?
poly(A) + H2O
-
Homo sapiens 5'-AMP + oligonucleotide
-
?
ss RNA + H2O
-
Homo sapiens 5'-phosphomononucleotides
-
?

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
Homo sapiens