Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA with a 2',3'-cyclic phosphate group | on the potential substrate molecule | Mus musculus | |
RNA with a terminal 3'-phosphate group | on the potential substrate molecule | Mus musculus | |
thymidine nucleotides | potent inhibitor, pTp at a concentration of 0.00004 M inhibits degradation of poly(A) by 60%, pTpT at a concentration of 0.0005 M inhibits degradation of poly(A) by 30% | Mus musculus | |
Urea | loss of 40% of activity in presence of 1.6 M, 60% in presence of 2.4 M and 75% in presence of 3.2 M urea | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Mus musculus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ss RNA + H2O | Mus musculus | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | acts in a processive manner | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast adenocarcinoma cell | - |
Mus musculus | - |
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
embryo | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-70°C, more than 50% of activity after more than a year | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 3'-phosphorylated RNA is not hydrolyzed | Mus musculus | ? | - |
? | |
poly(A) + H2O | - |
Mus musculus | 5'-AMP + oligonucleotide | - |
? | |
ss RNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in 0.1 M Tris-Cl, pH 8.0, for 15 min, loss of 20-30% of activity | Mus musculus |
50 | - |
in 0.1 M Tris-Cl, pH 8.0, for 60 min, loss of 40-60% of activity | Mus musculus |