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Literature summary for 3.1.13.1 extracted from
- An exoribonuclease from Saccharomyces cerevisiae: effect of modifications of 5'-end groups on the hydrolysis of substrates to 5'-mononucleotides (1978), Biochem. Biophys. Res. Commun., 81, 656-661.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-capped mRNA | - |
Saccharomyces cerevisiae |  |
| Rna with a terminal 5'-phosphate group | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| ribosome | - |
Saccharomyces cerevisiae | 5840 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | necessary for activity | Saccharomyces cerevisiae | |
| Mg2+ | optimum: 1.5 mM | Saccharomyces cerevisiae | |
| Mn2+ | necessary for activity | Saccharomyces cerevisiae | |
| Mn2+ | optimum: 0.3 mM, stimulation about two-thirds the rate with Mg2+ | Saccharomyces cerevisiae | |
| NH4+ | activating | Saccharomyces cerevisiae | |
| NH4+ | stimulates at pH 9.5 about 100%, optimum: 50 mM | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ss RNA + H2O | Saccharomyces cerevisiae | - |
5'-phosphomononucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 200fold | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | acts in a processive manner | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | capped mRNA is not hydrolyzed | Saccharomyces cerevisiae | ? | - |
? | |
| poly(A) + H2O | - |
Saccharomyces cerevisiae | 5'-AMP + oligonucleotide | - |
? | |
| ss RNA + H2O | - |
Saccharomyces cerevisiae | 5'-phosphomononucleotides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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