Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.11.6 extracted from

  • Repar, J.; Briski, N.; Buljubasic, M.; Zahradka, K.; Zahradka, D.
    Exonuclease VII is involved in "reckless" DNA degradation in UV-irradiated Escherichia coli (2013), Mutat. Res., 750, 96-104.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

General Information

General Information Comment Organism
physiological function inactivation of exonuclease VII by a mutation in its large subunit xseA contributes to attenuation of DNA degradation in UV-irradiated recA mutants. The xseA mutation itself has only a weak effect, however, it acts synergistically with the xonA or sbcD mutations, which inactivate exonuclease I and SbcCD nuclease, respectively, in suppressing reckless DNA degradation. The quadruple xseA xonA sbcD recA mutants show no sign of DNA degradation during post-irradiation incubation, suggesting that ExoVII, together with ExoI and SbcCD, plays a crucial role in regulating RecBCD-catalyzed chromosome degradation. These nucleases may act on double-strand DNA breaks to create blunt DNA ends, the preferred substrates for the RecBCD enzyme. In UV-irradiated recF recA+ cells, the xseA, xonA, and sbcD mutations do not affect RecBCD-mediated DNA repair, suggesting that ExoVII, ExoI and SbcCD nucleases are not essential for the initial targeting of RecBCD to double-strand NA breaks Escherichia coli