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Literature summary for 3.1.11.6 extracted from

  • Larrea, A.A.; Pedroso, I.M.; Malhotra, A.; Myers, R.S.
    Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima (2008), Nucleic Acids Res., 36, 5992-6003.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.6

Activating Compound

Activating Compound Comment Organism Structure
additional information acetate has no effect Escherichia coli
phosphate stimulates Escherichia coli
sulfate stimulates Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) coexpressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D235A/D240A is completely inactive, even in the presence of magnesium Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
EDTA completely inhibits Thermotoga maritima
additional information is not active in standard Escherichia coli ExoVII buffer. Acetate has no effect Thermotoga maritima
phosphate inhibits nuclease activity at 80°C; strongly inhibits, almost complete inhibition by 50 mM Thermotoga maritima
sulfate strongly inhibits, almost complete inhibition by 50 mM Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
KCl ExoVII activity is maximal at low salt concentrations (lower than 250 mM) Thermotoga maritima
Mg2+ robust nuclease activity at 80°C is magnesium dependent Thermotoga maritima
additional information manganese, nickel, cobalt and zinc have no effect Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified by gel filtration and on nickel-NTA column Thermotoga maritima
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified on nickel-NTA column Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
221
-
-
 Thermotoga maritima
267
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity Thermotoga maritima ?
-
 ?
additional information no significant activity on dsDNA. Active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity Thermotoga maritima ?
-
 ?
single-stranded DNA + H2O ExoVII activity is highly processive. It binds ssDNA ends and cleaves its substrate endonucleolytically, releasing oligonucleotide products of various sizes Thermotoga maritima ?
-
 ?
single-stranded DNA + H2O releases oligonucleotide products Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
More stoichiometry of small to large subunits regulates ExoVII activity Thermotoga maritima

Synonyms

Synonyms Comment Organism
exonuclease VII
-
 Escherichia coli
exonuclease VII
-
 Thermotoga maritima
XseA
-
 Thermotoga maritima
XseB
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 XseA/B homologs TM1768 and TM1769 show robust and maximal nuclease activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 XseA/B homologs TM1768 and TM1769 show robust nuclease activity Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Thermotoga maritima