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Literature summary for 3.1.11.5 extracted from

  • Pavankumar, T.; Sinha, A.; Ray, M.
    All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W (2010), PLoS ONE, 5, e9412.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D1118A site-directed mutagenesis, inactivation in the nuclease center of RecB Pseudomonas syringae
K229Q site-directed mutagenesis, inactivation of the ATPase active site of RecD Pseudomonas syringae
K29Q site-directed mutagenesis, inactivation of the ATPase active site of RecB Pseudomonas syringae
additional information disruption of genes in the recCBD operon and creation of DELTArecC, DELTArecB, DELTArecD, and DELTArecCBD strains of Pseudomonas syringae Lz4W Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
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three genes, recC, recB, and recD, in the recCBD operon
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Pseudomonas syringae Lz4W
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three genes, recC, recB, and recD, in the recCBD operon
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Synonyms

Synonyms Comment Organism
recBCD enzyme
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Pseudomonas syringae

General Information

General Information Comment Organism
malfunction individual null-mutations of all three genes, recC, recB, and recD, or the deletion of whole recCBD operon of Pseudomonas syringae, lead to growth inhibition at low temperature, and sensitivity to UV and mitomycin C. Viability of the mutant cells drops drastically at 4°C, and the mutants accumulate linear chromosomal DNA and shorter DNA fragments in higher amounts compared to 22°C Pseudomonas syringae
physiological function RecD associates with two other proteins RecB and RecC to produce RecBCD enzyme, which is involved in homologous recombination and DNA repair in many bacteria, including Escherichia coli. All three subunits of the RecBCDPs enzyme are essential for DNA repair and growth of Pseudomonas syringae at low temperatures of 4°C. The RecD requirement is only a function of the RecBCD complex in the bacterium. The RecBCD pathway protects the Antarctic bacterium from cold-induced DNA damages, and is critically dependent on the helicase activities of both RecB and RecD subunits, but not on the nuclease of RecBCDPs enzyme Pseudomonas syringae