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Literature summary for 3.1.11.5 extracted from

  • Doly, J.; Le Roscouet, D.; Anagnostopoulos, C.
    Substrate specificity and adenosine triphosphatase activity of the ATP-dependent deoxyribonuclease of Bacillus subtilis (1981), Eur. J. Biochem., 114, 493-499.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.2 mM and higher concentrations inhibits phosphodiester hydrolysis; neither the rate nor the extent of hydrolysis of single-stranded DNA nor ATP is affected Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
ATP
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
52500
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
62000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
70000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
81000
-
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
308000
-
gel filtration, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Bacillus subtilis
-
ADP + phosphate
-
?
double-stranded DNA + H2O Bacillus subtilis
-
single-stranded DNA fragments
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Bacillus subtilis ADP + phosphate
-
?
double-stranded DNA + H2O
-
Bacillus subtilis single-stranded DNA fragments
-
?

Subunits

Subunits Comment Organism
pentamer 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis