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Literature summary for 3.1.11.5 extracted from
- Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay (1989), Biochemistry, 28, 2863-2873.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | inhibitory to helicase activity | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000006 | - |
Double-stranded DNA | unwinding activity | Escherichia coli | |
| 0.13 | - |
ATP | unwinding activity | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitory on helicase activity in the presence of Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? | |
| double-stranded DNA + H2O | Escherichia coli | ATP-dependent helicase | single-stranded DNA fragments | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
| double-stranded DNA | unwinding in the presence of E. coli binding protein SSB or high levels of ATP | Escherichia coli | intermediates with single stranded regions | - |
? | |
| double-stranded DNA | no unwinding on replicative form DNA | Escherichia coli | intermediates with single stranded regions | - |
? | |
| double-stranded DNA + H2O | ATP-dependent helicase | Escherichia coli | single-stranded DNA fragments | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | 15000 base pairs of DNA per min at 25°C | Escherichia coli |
| 25 | 37 | 55800 base pairs of DNA per min at 37°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 250 | - |
ATP | DNA-unwinding activity | Escherichia coli | |
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