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Literature summary for 3.1.11.5 extracted from
- The ATP-dependent DNAase from Escherichia coli rorA: a nuclease with changed enzymatic properties (1975), Biochim. Biophys. Acta, 395, 446-454.
General Stability
| General Stability | Organism |
|---|---|
| DNA stabilizes against thermal denaturation | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | inhibitory at 40 mM and higher concentrations | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 270000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Escherichia coli | ATP-dependent exonuclease | single-stranded DNA fragments | - |
? | |
| single stranded DNA + H2O | Escherichia coli | ATP-dependent endonuclease | 5'-phosphomonoester oligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
rorA mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | slower degradation of UV-irradiated DNA | Escherichia coli | single-stranded DNA fragments | - |
? | |
| double-stranded DNA + H2O | ATP-dependent exonuclease | Escherichia coli | single-stranded DNA fragments | - |
? | |
| single stranded DNA + H2O | also endonuclease activity on circular DNA | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
| single stranded DNA + H2O | ATP-dependent endonuclease | Escherichia coli | 5'-phosphomonoester oligonucleotides | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
faster inactivation in presence of ATP | Escherichia coli |
| 42 | - |
complete stable in presence of DNA for 40 min | Escherichia coli |
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