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Literature summary for 3.1.11.5 extracted from

  • Oishi, M.
    An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination (1969), Proc. Natl. Acad. Sci. USA, 64, 1292-1299.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
3',5'-cyclic AMP less effective than ATP Escherichia coli
5'-AMP less effective than ATP Escherichia coli
adenine less effective than ATP Escherichia coli
adenosine less effective than ATP Escherichia coli
ADP less effective than ATP Escherichia coli
CTP less effective than ATP Escherichia coli
dCTP less effective than ATP Escherichia coli
GTP less effective than ATP Escherichia coli
S-adenosylmethionine less effective than ATP Escherichia coli
TTP less effective than ATP Escherichia coli
UTP less effective than ATP Escherichia coli

Application

Application Comment Organism
analysis possible role in genetic recombination Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
ATP
-
Escherichia coli
0.048
-
dATP
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli
Mn2+ less effective than Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
sedimentation, calculated Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli responsible for several steps in genetic recombination process single-stranded DNA fragments
-
?
double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
mutants lacking the recB or recC or recD gene
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
0°C, Tris, MgCl2, EDTA, mercaptoethanol, 1 d, 5-10% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Escherichia coli ADP + phosphate
-
?
double-stranded DNA + H2O acts on native, heat-denatured and glucosylated DNA Escherichia coli single-stranded DNA fragments
-
?
double-stranded DNA + H2O responsible for several steps in genetic recombination process Escherichia coli single-stranded DNA fragments
-
?
double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
Escherichia coli