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Literature summary for 3.1.11.3 extracted from

  • Oliver-Calixte, N.J.; Uba, F.I.; Battle, K.N.; Weerakoon-Ratnayake, K.M.; Soper, S.A.
    Immobilization of lambda exonuclease onto polymer micropillar arrays for the solid-phase digestion of dsDNAs (2014), Anal. Chem., 86, 4447-4454.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis solid-phase digestion of dsDNAs using immobilization of lambda exonuclease onto poly(methylmethacrylate) micropillars populated within a microfluidic device for the on-chip digestion. The efficiency for the catalysis of dsDNA digestion using lamba-exonuclease, including its processivity and reaction rate, are higher when the enzyme is attached to a solid support compared to the free solution digestion. A clipping rate of 1000 nucleotides per s can be obtained for the digestion of lambda-DNA (48.5 kbp) by lambda-exonuclease Lambdavirus lambda

Organism

Organism UniProt Comment Textmining
Lambdavirus lambda
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