Application | Comment | Organism |
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additional information | preparation of single-stranded DNA is an essential and important step in the combinatorial chemistry technique SELEX (Systematic Evolution of Ligands by EXponential enrichment) for in vitro selection of single-stranded DNA aptamers and numerous other molecular biology procedures, whereby single-stranded DNA generation by lambda exonuclease digestion is superior to other techniques. Important role for complete lambda exonuclease digestion of phosphorylated DNA strand plays the manufacturing of phosphorylated primer | synthetic construct |
Organism | UniProt | Comment | Textmining |
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synthetic construct | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | lambda exonuclease selectively digests the phosphorylated undesired strand of double-stranded DNA and produces single-stranded DNA in high yield and purity | synthetic construct | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
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Synonyms | Comment | Organism |
---|---|---|
lambda exonuclease | - |
synthetic construct |