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BRENDA support

Literature summary for 3.1.11.3 extracted from

  • Avci-Adali, M.; Paul, A.; Wilhelm, N.; Ziemer, G.; Wendel, H.P.
    Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation (2010), Molecules, 15, 1-11.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information preparation of single-stranded DNA is an essential and important step in the combinatorial chemistry technique SELEX (Systematic Evolution of Ligands by EXponential enrichment) for in vitro selection of single-stranded DNA aptamers and numerous other molecular biology procedures, whereby single-stranded DNA generation by lambda exonuclease digestion is superior to other techniques. Important role for complete lambda exonuclease digestion of phosphorylated DNA strand plays the manufacturing of phosphorylated primer synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O lambda exonuclease selectively digests the phosphorylated undesired strand of double-stranded DNA and produces single-stranded DNA in high yield and purity synthetic construct single-stranded DNA + 5'-phospho-2'-deoxynucleotides
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Synonyms

Synonyms Comment Organism
lambda exonuclease
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synthetic construct