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Literature summary for 3.1.11.3 extracted from
- Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage (2009), Anal. Chem., 81, 1383-1388.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a novel method for real-time monitoring of the activity and kinetics of T4 polynucleotide kinase (PNK) by use of a singly fluorophore-labeled DNA-hairpin smart probe (SP) coupled with lambda exonuclease (lambda exo) cleavage. | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
- |
Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | lambda exonuclease is unable to initiate DNA digestion at nicks or gaps | Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lambda exo | - |
Escherichia coli |
| lambda exonuclease | - |
Escherichia coli |
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