Any feedback?
Literature summary for 3.1.11.3 extracted from
- The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease (1971), J. Biol. Chem., 246, 2502-2512.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel fitration, SDS-PAGE | Escherichia coli |
| 24000 | - |
gel fitration, SDS-PAGE | Lambdavirus lambda |
| 28000 | - |
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Escherichia coli |
| 28000 | - |
alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Lambdavirus lambda |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Escherichia coli | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? |  |
| double-stranded DNA + H2O | Lambdavirus lambda | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| single-stranded DNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? | |
| single-stranded DNA + H2O | Lambdavirus lambda | - |
5'-phosphomononucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Escherichia coli infected with phage lambda only | - |
| Lambdavirus lambda | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Lambdavirus lambda |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | - |
Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| double-stranded DNA + H2O | - |
Lambdavirus lambda | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| double-stranded DNA + H2O | no attack at single-stranded breaks within DNA-duplex | Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| double-stranded DNA + H2O | no attack at single-stranded breaks within DNA-duplex | Lambdavirus lambda | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| single-stranded DNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
| single-stranded DNA + H2O | - |
Lambdavirus lambda | 5'-phosphomononucleotides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Escherichia coli |
| dimer | alpha,beta, 1 * 24000 + 1 * 28000, exonuclease and beta protein as subunits, SDS-PAGE at the presence of urea | Lambdavirus lambda |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
no activity at DNA nicks | Escherichia coli |
| 7.5 | - |
no activity at DNA nicks | Lambdavirus lambda |
| 9.6 | - |
no activity at DNA nicks | Escherichia coli |
| 9.6 | - |
no activity at DNA nicks | Lambdavirus lambda |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
