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Literature summary for 3.1.11.3 extracted from
- An exonuclease induced by bacteriophage lambda. I. Preparation of the crystalline enzyme (1967), J. Biol. Chem., 242, 672-678.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| by ammonium sulfate precepitation | Escherichia coli |
| by ammonium sulfate precepitation | Lambdavirus lambda |
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing causes loss of activity | Escherichia coli |
| repeated freezing and thawing causes loss of activity | Lambdavirus lambda |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 31% inhibition at 50 mM | Escherichia coli |  |
| KCl | 31% inhibition at 50 mM | Lambdavirus lambda | |
| NaCl | 82% inhibition at 50 mM | Escherichia coli | |
| NaCl | 82% inhibition at 50 mM | Lambdavirus lambda | |
| p-chloromercuribenzoate | - |
Escherichia coli | |
| p-chloromercuribenzoate | - |
Lambdavirus lambda | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Lambdavirus lambda | |
| Mn2+ | divalent cation: Mn2+ or Mg2+ required | Escherichia coli | |
| Mn2+ | divalent cation: Mn2+ or Mg2+ required | Lambdavirus lambda | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Escherichia coli | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| double-stranded DNA + H2O | Lambdavirus lambda | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Escherichia coli infected with phage lambda only | - |
| Lambdavirus lambda | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| subfractions upon gradient chromatography: IVa, IVb, phage-lambda induced enzyme | Escherichia coli |
| subfractions upon gradient chromatography: IVa, IVb, phage-lambda induced enzyme | Lambdavirus lambda |
| to homogeneity | Escherichia coli |
| to homogeneity | Lambdavirus lambda |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in solution, 1 year, 10% loss of activity | Escherichia coli |
| -20°C, in solution, 1 year, 10% loss of activity | Lambdavirus lambda |
| 0°C, in solution, 2 months, little loss of activity | Escherichia coli |
| 0°C, in solution, 2 months, little loss of activity | Lambdavirus lambda |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | - |
Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
| double-stranded DNA + H2O | - |
Lambdavirus lambda | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
complete inactivation after 5 min without substrate | Escherichia coli |
| 60 | - |
complete inactivation after 5 min without substrate | Lambdavirus lambda |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | 9.5 | - |
Escherichia coli |
| 9.2 | 9.5 | - |
Lambdavirus lambda |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10.2 | pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity | Escherichia coli |
| 7 | 10.2 | pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity | Lambdavirus lambda |
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