Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | required | Escherichia coli | |
dithiothreitol | required | Escherichia phage T7 | |
KCl | stimulates at 20 mM | Escherichia coli | |
KCl | stimulates at 20 mM | Escherichia phage T7 |
General Stability | Organism |
---|---|
stability enhanced by dithiothreitol | Escherichia coli |
stability enhanced by dithiothreitol | Escherichia phage T7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Escherichia phage T7 | |
Mg2+ | optimal concentration 5 mM | Escherichia coli | |
Mg2+ | optimal concentration 5 mM | Escherichia phage T7 | |
Mn2+ | divalent cation: Mn2+ or Mg2+ required | Escherichia coli | |
Mn2+ | divalent cation: Mn2+ or Mg2+ required | Escherichia phage T7 | |
Mn2+ | optimal concentration 1 mM | Escherichia coli | |
Mn2+ | optimal concentration 1 mM | Escherichia phage T7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | Escherichia phage T7 | - |
single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
Escherichia coli infected with phage lambda only | - |
Escherichia phage T7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
partial | Escherichia phage T7 |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity | Escherichia coli |
-20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity | Escherichia phage T7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | - |
Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | - |
Escherichia phage T7 | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | degradation also at single-stranded breaks within DNA-duplex | Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | degradation also at single-stranded breaks within DNA-duplex | Escherichia phage T7 | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | degrades double-stranded native DNA | Escherichia coli | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? | |
double-stranded DNA + H2O | degrades double-stranded native DNA | Escherichia phage T7 | single-stranded DNA + 5'-phospho-2'-deoxynucleotides | - |
? |