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Literature summary for 3.1.11.2 extracted from

  • Zhang, H.; Lin, Z.; Su, X.
    Label-free detection of exonuclease III by using dsDNA-templated copper nanoparticles as fluorescent probe (2015), Talanta, 131, 59-63 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NSU2
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Source Tissue

Source Tissue Comment Organism Textmining
serum
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Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information exonuclease III (ExoIII) is a sequence-independent 3'-5' exonuclease, that can catalyze the stepwise removal of mononucleotides from DNA duplexes in the direction from blunt or recessed 3'-termini to 5'-termini, while it is unable to catalyze the removal of bases from single-stranded DNA or DNA duplexes with a protruding 3' end. Establishment of a simple, sensitive, low-cost and label-free method to detect the activity of exonuclease III (Exo III) by using double-strand DNA (dsDNA)-templated copper nanoparticles as fluorescent probe. Design of the label-free fluorescent probe for Exo III activity detection. The sensing system contains two completely complementary nucleic acid strands, which are simply designed based on DNA hybridization. The hybridized dsDNA is introduced to act as both templates for CuNPs formation and substrate of Exo III. Fluorescent Cu nanoparticles (NPs) with maximum emission wavelength of 575 nm are formed by using double-strand DNA (dsDNA) as templates. Upon the addition of Exo III, the dsDNA templates are digested from 3' to 5', and the formation of fluorescent Cu-NPs is inhibited. Thus, the fluorescence intensity of dsDNA-Cu-NPs decreases. This method exhibits a low detection limit of 0.02 U/ml for Exo III. Compared with the previous reports, this method does not need complex DNA sequence design, fluorescence dye label and sophisticated experimental techniques, mechanism and method optimization, detailed overview Homo sapiens ?
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Synonyms

Synonyms Comment Organism
exo III
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Homo sapiens
exonuclease III
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Homo sapiens

General Information

General Information Comment Organism
physiological function exonuclease III (Exo III) is a sequence-independent 3'-5' exonuclease Homo sapiens