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Literature summary for 3.1.11.2 extracted from
- Enzymatically amplified surface plasmon resonance imaging detection of DNA by exonuclease III digestion of DNA microarrays (2005), Anal. Chem., 77, 5096-5100.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | enzymatically amplified surface plasmon resonance imaging detection of DNA by exonuclease III digestion of DNA microarrays. Through the use of ExoIII in conjunction with DNA microarrays, a 100-1000 improvement in the detection limit for the multiplexed surface plasmon resonance imaging detection of 16-mer oligonucleotides is achieved. This enhancement is not as great as RNase H amplification with RNA microarrays. The major advantage of ExoIII amplification compared to RNase H is that the additional difficulty in preparing and handling RNA microarrays is avoided. The ExoIII enzymatic amplification process can be used with the more robust and cost-effective DNA microarrays that are currently applied in research areas such as gene analysis and medical diagnostics. Most DNA sequences can be detected since the activity of ExoIII is not sequence dependent | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | - |
Escherichia coli | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exonuclease III | - |
Escherichia coli |
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