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Literature summary for 3.1.11.2 extracted from

  • Sun, W.; Nicholson, A.W.
    Mechanism of action of Escherichia coli ribonuclease III. Stringent chemical requirement for the glutamic acid 117 side chain and Mn2+ rescue of the Glu117Asp mutant (2001), Biochemistry, 40, 5102-5110.
    View publication on PubMed
Search for more literature for 3.1.11.2

Protein Variants

Protein Variants Comment Organism
E117D mutant is unable to cleave substrate in vitro without affecting substrate binding, substitution of Mn2+ for Mg2+ increases the activity Escherichia coli
E117Q mutant is unable to cleave substrate in vitro but can bind substrate, substitution of Mn2+ for Mg2+ does not increase the activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ at concentrations above 5 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, a high concentration of Mg2+ does not inhibit enzyme nor relieve the Mn2+-dependent inhibition Escherichia coli
Mn2+ the optimal concentration is 1 mM, while concentrations above 5 mM are inhibitory Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, SDS-PAGE, wild-type and mutant enzymes E117Q and E117D Escherichia coli
58000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes are purified as (His)6-tagged species Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 29000, SDS-PAGE, wild-type and mutant enzymes E117Q and E117D Escherichia coli

Synonyms

Synonyms Comment Organism
ribonuclease III
-
 Escherichia coli
RNase III
-
 Escherichia coli