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Literature summary for 3.1.11.2 extracted from
- Nucleotide sequence analysis of deoxyribonucleic acid. VII. Characterization of Escherichia coli exonuclease III activity for possible use in terminal nucleotide sequence analysis of duplex deoxyribonucleic acid (1972), J. Biol. Chem., 247, 4661-4668.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | analysis of 3'-terminal nucleotide sequences of duplex DNA. Under selected conditions the enzyme can be used to remove a small defined number of nucleotides from each 3'-terminus of duplex DNA | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | at 5°C in a high salt concentration and a ratio of enzyme to DNA 3'-terminus of one, the enzyme catalyzes the hydrolysis of approximately six nucleotides from each 3'-terminus of the duplex DNA. When the ratio of exonuclease to DNA 3'-terminus is increased to two approximately 12 nucleotides are hydrolyzed from each 3'-terminus | Escherichia coli | additional information | - |
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