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Literature summary for 3.1.11.2 extracted from

  • Roychoudhury, R.; Wu, R.
    Novel properties of Escherichia coli exonuclease III (1977), J. Biol. Chem., 252, 4786-4789.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
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Escherichia coli additional information the size of the products after 3'-5' hydrolysis is temperature-dependent. At 25°C the enzyme can hydrolyze a polynucleotide chain up to the last 5'-terminal dinucleotide. A gradation of higher 5'-terminal oligonucleotides of defined chain lengths is produced after limit digestion by the enzyme when the temperature is raised from 25°C to 60°C ?
additional information the enzyme can cleave a single or two consecutive ribonucleotides from base-paired or mismatched ribonucleotides Escherichia coli ?
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