Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.11.1 extracted from

  • Lawler, J.L.; Mukherjee, P.; Coen, D.M.
    Herpes simplex virus 1 DNA polymerase RNase H activity acts in a 3'-to-5' direction and is dependent on the 3'-to-5' exonuclease active site (2018), J. Virol., 92, e01813-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.1

Protein Variants

Protein Variants Comment Organism
D368A the exonuclease-deficient mutant is drastically impaired for 3'-to-5' exonuclease activity, with no activity detected even at high enzyme-to-DNA substrate ratios Human alphaherpesvirus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 8 mM used in assay conditions Human alphaherpesvirus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicked DNA containing mismatches + H2O Human alphaherpesvirus 1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography and heparin column chromatography Human alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-FAM-labeled double stranded DNA containing mismatches + H2O
-
 Human alphaherpesvirus 1 ?
-
 ?
6-FAM-labeled RNA-DNA hairpin containing mismatches + H2O
-
 Human alphaherpesvirus 1 ?
-
 ?
nicked DNA containing mismatches + H2O
-
 Human alphaherpesvirus 1 ?
-
 ?

Synonyms

Synonyms Comment Organism
3'-to-5' exonuclease
-
 Human alphaherpesvirus 1
Pol catalytic subunit of Herpes simplex virus 1 DNA polymerase Human alphaherpesvirus 1