Cloned (Comment) | Organism |
---|---|
expression of hEXO1 in Spodoptera frugiperda Sf9 insect cells using the baculovirus vector system | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mn2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | hEXO1 does not exhibit endonuclease activity on 5'-flaps bearing structures formed by CTG or CGG repeats, although it can excise these substrates. hEXO1 is not affected by the stem-loops formed by CTG repeats interrupting duplex regions adjacent to 5'-flaps, but the enzymes is inhibited by G4 structures formed by CGG repeats in analogous positions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hEXO1 does not exhibit endonuclease activity on 5'-flaps bearing structures formed by CTG or CGG repeats, although it can excise these substrates. hEXO1 is not affected by the stem-loops formed by CTG repeats interrupting duplex regions adjacent to 5'-flaps, but the enzymes is inhibited by G4 structures formed by CGG repeats in analogous positions | Homo sapiens | ? | - |
? | |
additional information | hEXO1 exhibits both flap endonucleolytic cleavage activity and exonucleolytic activity on the substrates carrying a duplex region or a duplex region interrupted by a (CTG)8 repeat, but not on the substrate in which a (CGG)6 repeat interrupted the duplex | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Homo sapiens |
exonuclease 1 | - |
Homo sapiens |
More | EXO1 belongs to the RAD2 family nucleases, FEN1 (Flap endonuclease 1) and EXO1 (exonuclease 1) | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | EXO1 can eliminate structures formed by trinucleotide repeats in the course of DNA replication | Homo sapiens |