Cloned (Comment) | Organism |
---|---|
murine NIH3T3 cells are transiently cotransfected with plasmids expressing CFP-PCNA and YFP-hEXO1, expression of wild-type and mutant N-terminal YFP-tagged hEXO1 fusion proteins in Escherichia coli strain BL21(DE3), in vitro transcription translation of hEXO1, plasmid pcDNA3.1A/myc-His(-)A-hEXO1 expressing COOH-terminal his6 and myc epitope tagged hEXO1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F506A/F507A | site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution | Homo sapiens |
Q154A/Y157A | site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution | Homo sapiens |
Q285A/F288A | site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | sub-nuclear localization of hEXO1 in replication foci during S-phase progression and in response to laser-induced DNA double strand breaks, hEXO1 is rapidly recruited to DNA double strand breaks, mechanism, overview. Co-localization with proliferating cell nuclear antigen. Mapping of region on hEXO1 required for recruitment to DNA double strand breaks | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UQ84 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | binding between PCNA and hEXO1 peptides p22 (aa 783-804) and p16 (aa 498-513) and isothermal titration calorimetry, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Homo sapiens |
exonuclease 1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | mapping of region on hEXO1 required for recruitment to DNA double strand breaks, mechanism of hEXO1 recruitment, overview | Homo sapiens |
physiological function | human exonuclease 1 is a multifunctional protein that plays roles in DNA metabolism, including replication, recombination and repair, substantiated by its interactions with proliferating cell nuclear antigen, DNA helicases BLM and WRN, and several DNA mismatch repair proteins | Homo sapiens |