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Literature summary for 3.1.11.1 extracted from

  • Liberti, S.E.; Andersen, S.D.; Wang, J.; May, A.; Miron, S.; Perderiset, M.; Keijzers, G.; Nielsen, F.C.; Charbonnier, J.B.; Bohr, V.A.; Rasmussen, L.J.
    Bi-directional routing of DNA mismatch repair protein human exonuclease 1 to replication foci and DNA double strand breaks (2011), DNA Repair, 10, 73-86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
murine NIH3T3 cells are transiently cotransfected with plasmids expressing CFP-PCNA and YFP-hEXO1, expression of wild-type and mutant N-terminal YFP-tagged hEXO1 fusion proteins in Escherichia coli strain BL21(DE3), in vitro transcription translation of hEXO1, plasmid pcDNA3.1A/myc-His(-)A-hEXO1 expressing COOH-terminal his6 and myc epitope tagged hEXO1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
F506A/F507A site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution Homo sapiens
Q154A/Y157A site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution Homo sapiens
Q285A/F288A site-directed mutagenesis, the expression of the mutant interferes with S-phase distribution Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus sub-nuclear localization of hEXO1 in replication foci during S-phase progression and in response to laser-induced DNA double strand breaks, hEXO1 is rapidly recruited to DNA double strand breaks, mechanism, overview. Co-localization with proliferating cell nuclear antigen. Mapping of region on hEXO1 required for recruitment to DNA double strand breaks Homo sapiens 5634
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UQ84
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information binding between PCNA and hEXO1 peptides p22 (aa 783-804) and p16 (aa 498-513) and isothermal titration calorimetry, overview Homo sapiens ?
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Synonyms

Synonyms Comment Organism
Exo1
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Homo sapiens
exonuclease 1
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Homo sapiens

General Information

General Information Comment Organism
additional information mapping of region on hEXO1 required for recruitment to DNA double strand breaks, mechanism of hEXO1 recruitment, overview Homo sapiens
physiological function human exonuclease 1 is a multifunctional protein that plays roles in DNA metabolism, including replication, recombination and repair, substantiated by its interactions with proliferating cell nuclear antigen, DNA helicases BLM and WRN, and several DNA mismatch repair proteins Homo sapiens