Crystallization (Comment) | Organism |
---|---|
nine crystal forms at resolutions between 1.4 and 2.8 A. The catalytic triad comprises Ser232, Asp340 and His389 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylmethanesulfonylfluoride | strong inhibition of 4-nitrophenylacetate hydrolysis | Homo sapiens | |
Triton X-100 | strong inhibition of 4-nitrophenylacetate hydrolysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VUX3 | - |
- |
Homo sapiens | Q6P988 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
? | |
additional information | no measurable activity on representative sulfatase, phosphatase, phospholipase C or amidase/protease substrates | Homo sapiens | ? | - |
? | |
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H2O | - |
Drosophila melanogaster | [Wnt]-L-serine + (9Z)-hexadec-9-enoate | Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins | ? | |
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H2O | - |
Homo sapiens | [Wnt]-L-serine + (9Z)-hexadec-9-enoate | Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins | ? |
General Information | Comment | Organism |
---|---|---|
physiological function | Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor. Glycosaminoglycan binding sites on Notum probably help to co-localize with Wnt proteins. A large hydrophobic pocket at the active site accommodates palmitoleate | Drosophila melanogaster |
physiological function | Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor. Glycosaminoglycan binding sites on Notum probably help to co-localize with Wnt proteins. A large hydrophobic pocket at the active site accommodates palmitoleate | Homo sapiens |