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show all sequences of 3.1.1.90

Aromatic residues in the substrate cleft of RPE65 protein govern retinol isomerization and modulate its progression

Chander, P.; Gentleman, S.; Poliakov, E.; Redmond, T.; J. Biol. Chem. 287, 30552-30559 (2012)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F103I
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103L
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103W
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103Y
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F312L
mutant shows severly impaired enzymatic activity
Mus musculus
F312W
mutant shows severly impaired enzymatic activity
Mus musculus
F312Y
mutant shows severly impaired enzymatic activity
Mus musculus
F418A
mutant shows severly impaired enzymatic activity
Mus musculus
F418L
mutant shows severly impaired enzymatic activity
Mus musculus
F418W
mutant shows severly impaired enzymatic activity
Mus musculus
F418Y
mutant shows severly impaired enzymatic activity
Mus musculus
F526A
mutant shows a increased production level of 13-cis-retinol compared to wild-type
Mus musculus
F526L
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F526W
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F526Y
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F61A
mutant shows severly impaired enzymatic activity
Mus musculus
F61L
mutant shows severly impaired enzymatic activity
Mus musculus
F61W
mutant shows severly impaired enzymatic activity
Mus musculus
F61Y
mutant shows severly impaired enzymatic activity
Mus musculus
Y338A
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338F
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338L
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338W
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans retinyl ester + H2O
-
732066
Mus musculus
13-cis retinol + ?
-
-
-
-
all-trans retinyl ester + H2O
-
732066
Mus musculus
11-cis retinol + ?
-
-
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F103I
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103L
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103W
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F103Y
mutant produces reduced amounts of 13-cis-retinol and reduced production of 11-cis-retinol compared to wild-type
Mus musculus
F312L
mutant shows severly impaired enzymatic activity
Mus musculus
F312W
mutant shows severly impaired enzymatic activity
Mus musculus
F312Y
mutant shows severly impaired enzymatic activity
Mus musculus
F418A
mutant shows severly impaired enzymatic activity
Mus musculus
F418L
mutant shows severly impaired enzymatic activity
Mus musculus
F418W
mutant shows severly impaired enzymatic activity
Mus musculus
F418Y
mutant shows severly impaired enzymatic activity
Mus musculus
F526A
mutant shows a increased production level of 13-cis-retinol compared to wild-type
Mus musculus
F526L
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F526W
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F526Y
mutant shows a decreased production level of 11-cis-retinol compared to wild-type
Mus musculus
F61A
mutant shows severly impaired enzymatic activity
Mus musculus
F61L
mutant shows severly impaired enzymatic activity
Mus musculus
F61W
mutant shows severly impaired enzymatic activity
Mus musculus
F61Y
mutant shows severly impaired enzymatic activity
Mus musculus
Y338A
mutant produces increased amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338F
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338L
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Y338W
mutant produces similar amounts of 13-cis-retinol but reduced production of 11-cis-retinol compared to wild-type
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans retinyl ester + H2O
-
732066
Mus musculus
13-cis retinol + ?
-
-
-
-
all-trans retinyl ester + H2O
-
732066
Mus musculus
11-cis retinol + ?
-
-
-
-
Other publictions for EC 3.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729213
Takahashi
Identification of the key resi ...
Danio rerio
Biochemistry
51
4217-4225
2012
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1
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10
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1
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2
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1
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4
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1
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10
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1
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1
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4
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2
2
-
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-
732066
Chander
Aromatic residues in the subst ...
Mus musculus
J. Biol. Chem.
287
30552-30559
2012
-
-
-
-
23
-
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-
1
-
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-
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2
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23
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2
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714991
Takahashi
An enzymatic mechanism for gen ...
Danio rerio
FEBS J.
278
973-987
2011
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1
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1
1
1
1
1
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1
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1
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2
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1
1
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1
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1
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1
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1
1
1
1
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1
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2
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1
1
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1
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1
1
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