BRENDA - Enzyme Database show
show all sequences of 3.1.1.90

An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain

Takahashi, Y.; Moiseyev, G.; Chen, Y.; Farjo, K.; Nikolaeva, O.; Ma, J.X.; FEBS J. 278, 973-987 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in 293A-LRAT and 293T
Danio rerio
Inhibitors
Inhibitors
Commentary
Organism
Structure
bipyridine
complete inhibition at 1 mM
Danio rerio
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Danio rerio
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on
Danio rerio
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Danio rerio
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Danio rerio
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
high expression
Danio rerio
-
eye
low expression
Danio rerio
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans retinyl ester + H2O
-
714991
Danio rerio
13-cis retinol + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00044
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in 293A-LRAT and 293T
Danio rerio
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
bipyridine
complete inhibition at 1 mM
Danio rerio
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Danio rerio
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on
Danio rerio
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Danio rerio
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
high expression
Danio rerio
-
eye
low expression
Danio rerio
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans retinyl ester + H2O
-
714991
Danio rerio
13-cis retinol + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00044
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
General Information
General Information
Commentary
Organism
physiological function
the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain
Danio rerio
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain
Danio rerio
Other publictions for EC 3.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729213
Takahashi
Identification of the key resi ...
Danio rerio
Biochemistry
51
4217-4225
2012
-
-
1
-
10
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
732066
Chander
Aromatic residues in the subst ...
Mus musculus
J. Biol. Chem.
287
30552-30559
2012
-
-
-
-
23
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
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-
-
-
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-
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-
-
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-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714991
Takahashi
An enzymatic mechanism for gen ...
Danio rerio
FEBS J.
278
973-987
2011
-
-
1
-
-
-
1
1
1
1
1
-
-
1
-
-
1
-
-
2
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
1
1
1
-
-
-
-
1
-
2
-
-
1
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-