Literature summary for 3.1.1.90 extracted from

Takahashi, Y.; Moiseyev, G.; Chen, Y.; Farjo, K.; Nikolaeva, O.; Ma, J.X.
An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain (2011), FEBS J., 278, 973-987.

Search for more literature for 3.1.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in 293A-LRAT and 293T	Danio rerio

Inhibitors

Inhibitors	Comment	Organism	Structure
bipyridine	complete inhibition at 1 mM	Danio rerio

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0026	-	all-trans retinyl ester	pH and temperature not specified in the publication	Danio rerio

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Danio rerio	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	dependent on	Danio rerio

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
61000	-	x * 61000, recombinant enzyme, SDS-PAGE	Danio rerio

Organism

Organism	UniProt	Comment	Textmining
Danio rerio	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Danio rerio

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	high expression	Danio rerio	-
eye	low expression	Danio rerio	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
all-trans retinyl ester + H2O	-	Danio rerio	13-cis retinol + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 61000, recombinant enzyme, SDS-PAGE	Danio rerio

Synonyms

Synonyms	Comment	Organism
13-cis isomerohydrolase	-	Danio rerio
13-cis retinoid-specific isomerohydrolase	-	Danio rerio
13cIMH	-	Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00044	-	all-trans retinyl ester	pH and temperature not specified in the publication	Danio rerio

General Information

General Information	Comment	Organism
physiological function	the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain	Danio rerio

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Release 2023.1 (January 2023)