Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | more than 75% enzyme activity inhibition | Sphingobium sp. | |
Cu2+ | more than 75% enzyme activity inhibition | Sphingobium sp. | |
Hg2+ | more than 75% enzyme activity inhibition | Sphingobium sp. | |
malathion | 0.5 mM, 96% inhibition | Sphingobium sp. | |
p-chloromercuribenzoic acid | 0.5 mM, complete inhibition | Sphingobium sp. | |
phenylmethylsulfonyl fluoride | 0.5 mM, 99% inhibition | Sphingobium sp. | |
SDS | 10 mM, 90% inhibition | Sphingobium sp. | |
Zn2+ | more than 75% enzyme activity inhibition | Sphingobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Sphingobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. | - |
- |
- |
Sphingobium sp. JZ-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.042 | - |
pH and temperature not specified in the publication | Sphingobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bifenthrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate | - |
? | |
bifenthrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. JZ-2 | (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate | - |
? | |
cypermethrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
cypermethrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. JZ-2 | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
deltamethrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
deltamethrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. JZ-2 | 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
fenpropathrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide | cyano-3-phenoxybenzyl alcohol is unstable and is transformed spontaneously to 3-phenoxybenzaldehyde | ? | |
fenpropathrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. JZ-2 | 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide | cyano-3-phenoxybenzyl alcohol is unstable and is transformed spontaneously to 3-phenoxybenzaldehyde | ? | |
fenvalerate + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide | - |
? | |
fenvalerate + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. JZ-2 | 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide | - |
? | |
permethrin + H2O | fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin | Sphingobium sp. | 3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Sphingobium sp. |
Synonyms | Comment | Organism |
---|---|---|
pyrethroid-hydrolyzing enzyme | - |
Sphingobium sp. |
pytH | - |
Sphingobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sphingobium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
fairly stable below | Sphingobium sp. |
65 | - |
nearly completely inactivated | Sphingobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Sphingobium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | more than 75% of maximal activity at pH 6.0 and at pH 8.0 | Sphingobium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | stable | Sphingobium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactors or coenzymes are required for the pyrethroid-hydrolysis activity | Sphingobium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sphingobium sp. | isoelectric focusing, pH-range: 3.5-10 | - |
4.85 |