Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 22% activation, Phedase type 1 | Raphanus sativus | |
EDTA | 1 mM, 9% activation, Phedase type 2 | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methanol | 1 M, 20% inhibition, Phedase type 1; 1 M, 20% inhibition, Phedase type 2 | Raphanus sativus | |
N-ethylmaleimide | 1 mM, 11% inhibition, Phedase type 1 | Raphanus sativus | |
phenylmethylsulfonic fluoride | 1 mM, 26.4% inhibition, Phedase type 2; 1 mM, 61% inhibition, Phedase type 1 | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0141 | - |
pheophorbide a | 30°C, pH 7.0, Phedase type 1 | Raphanus sativus | |
0.0151 | - |
pheophorbide a | 30°C, pH 7.0, Phedase type 2 | Raphanus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, Phedase type 1, SDS-PAGE | Raphanus sativus |
113000 | - |
Phedase type 1, gel filtration | Raphanus sativus |
113000 | - |
Phedase type 2, gel filtration | Raphanus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pheophorbide a + H2O | Raphanus sativus | Phedase type 1 is senescence-induced | pyropheophorbide a + methanol + CO2 | - |
? | |
pheophorbide a + H2O | Raphanus sativus | Phedase type 2 is constitutively produced | pyropheophorbide a + methanol + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
low activity | - |
Brassica napus | - |
- |
- |
Brassica oleracea | - |
- |
- |
Brassica rapa subsp. chinensis | - |
- |
- |
Brassica rapa subsp. oleifera | - |
- |
- |
Brassica rapa subsp. pekinensis | - |
low activity | - |
Chenopodium album | - |
- |
- |
Hypolepis punctata | - |
- |
- |
Nasturtium officinale | - |
- |
- |
no activity in Camellia sinensis | - |
- |
- |
no activity in Chlamydomonas reinhardtii | - |
- |
- |
no activity in Chrysanthemum coronarium | - |
- |
- |
no activity in Corchorus olitorius | - |
- |
- |
no activity in Equisetum arvense | - |
- |
- |
no activity in Ginkgo biloba | - |
- |
- |
no activity in Hordeum vulgare | - |
- |
- |
no activity in Lepisorus thunbergianus | - |
- |
- |
no activity in Perilla frutescens | - |
- |
- |
no activity in Petroselinum sativum | - |
- |
- |
no activity in Pisum sativum | - |
- |
- |
no activity in Rumex acetosa | - |
- |
- |
no activity in Spinacia oleracea | - |
- |
- |
Raphanus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Phedase type 1 | Raphanus sativus |
Phedase type 2 | Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Raphanus sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pheophorbide a + H2O | Phedase type 1 is senescence-induced | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
pheophorbide a + H2O | Phedase type 2 is constitutively produced | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
pheophorbide a + H2O | the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 1 | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
pheophorbide a + H2O | the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 2 | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Raphanus sativus |
dimer | 2 * 57000, Phedase type 1, SDS-PAGE | Raphanus sativus |
Synonyms | Comment | Organism |
---|---|---|
phedase | - |
Arabidopsis thaliana |
phedase | - |
Brassica napus |
phedase | - |
Raphanus sativus |
phedase | - |
Brassica oleracea |
phedase | - |
Brassica rapa subsp. oleifera |
phedase | - |
Brassica rapa subsp. pekinensis |
phedase | - |
Nasturtium officinale |
phedase | - |
Chenopodium album |
phedase | - |
Brassica rapa subsp. chinensis |
phedase | - |
Hypolepis punctata |
Phedase type 1 | - |
Raphanus sativus |
Phedase type 2 | - |
Raphanus sativus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
50% loss of activity at 67.5°C, Phedase type 1 | Raphanus sativus |
68 | - |
50% loss of activity at 67.5°C, Phedase type 2 | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
Phedase type 2 | Raphanus sativus |
7 | 7.5 | Phedase type 1 | Raphanus sativus |