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Literature summary for 3.1.1.81 extracted from

  • Dong, W.; Zhu, J.; Guo, X.; Kong, D.; Zhang, Q.; Zhou, Y.; Liu, X.; Zhao, S.; Ruan, Z.
    Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1 (2018), Sci. Rep., 8, 6013 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Activating Compound

Activating Compound Comment Organism Structure
protease K activity is enhanced up to 109% after preincubations with trypsin and protease K at 37°C for 30 and 60 min Kurthia huakuii
Trypsin activity is enhanced up to 109% after preincubations with trypsin and protease K at 37°C for 30 and 60 min Kurthia huakuii

Application

Application Comment Organism
drug development the enzyme significantly inhibits the biofilm formation and attenuates extracellular proteolytic activity and pyocyanin production of Pseudomonas aeruginosa PAO1, which indicates the potential application of AiiK as a biocontrol agent or an anti-pathogenic drug Kurthia huakuii
pharmacology the enzyme significantly inhibits the biofilm formation and attenuates extracellular proteolytic activity and pyocyanin production of Pseudomonas aeruginosa PAO1, which indicates the potential application of AiiK as a biocontrol agent or an anti-pathogenic drug Kurthia huakuii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Kurthia huakuii

Inhibitors

Inhibitors Comment Organism Structure
alpha-chymotrypsin the enzyme maintains 87% and 101% activity after preincubations with alpha-chymotrypsin at 37°C for 30 min and 60 min Kurthia huakuii
Ca2+ 1 mM, 30% inhibition. 5 mM, 45% inhibition Kurthia huakuii
Co2+ 5 mM, 50% inhibition Kurthia huakuii
Cu2+ 1 mM, complete inhibition Kurthia huakuii
EDTA 5 mM, 50% inhibition Kurthia huakuii
Fe2+ 5 mM, 25% inhibition Kurthia huakuii
Mg2+ 5 mM, 10% loss of activity Kurthia huakuii
Ni2+ 5 mM, 50% inhibition Kurthia huakuii
Zn2+ 1 mM, 20% inhibition. 5 mM, 90% inhibition Kurthia huakuii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 N-tetradecanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
0.066
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
0.204
-
 N-hexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
0.315
-
 N-decanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 1.25fold activation Kurthia huakuii
Mn2+ 1 mM, 1.4fold activation Kurthia huakuii
Ni2+ 1 mM, 1.5fold activation Kurthia huakuii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE, purified fusion protein containing complex soluble tags (Trx-Tag, His-Tag and S-Tag) Kurthia huakuii

Organism

Organism UniProt Comment Textmining
Kurthia huakuii
-
-
-
Kurthia huakuii LAM0618T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kurthia huakuii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii LAM0618T N-3-oxohexanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii LAM0618T N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii LAM0618T N-hexanoyl-L-homoserine
-
 ?
N-tetradecanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii N-tetradecanoyl-L-homoserine
-
 ?
N-tetradecanoyl-L-homoserine lactone + H2O
-
 Kurthia huakuii LAM0618T N-tetradecanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 31760, calculated from sequence Kurthia huakuii

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Kurthia huakuii
AiiK
-
 Kurthia huakuii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Kurthia huakuii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 65 5°C: about 40% of maximal activity, 65°C: about 35% of maximal activity Kurthia huakuii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 about 50% loss of activity after 4 h, about 80% loss of activity after 12 h Kurthia huakuii
45
-
 about 50% loss of activity after 4 h, about 90% loss of activity after 12 h Kurthia huakuii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 N-tetradecanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
0.168
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
0.649
-
 N-hexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
1.321
-
 N-decanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Kurthia huakuii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 AiiK is stable at pH values ranging from 6 to 8, maintaining over 70% activity after preincubations for 30 min at 25°C Kurthia huakuii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.92
-
 N-tetradecanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
2.7
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
3.2
-
 N-hexanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii
3.9
-
 N-decanoyl-L-homoserine lactone pH 7.4, 25°C Kurthia huakuii