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Literature summary for 3.1.1.81 extracted from

  • Bergonzi, C.; Schwab, M.; Naik, T.; Daude, D.; Chabriere, E.; Elias, M.
    Structural and biochemical characterization of AaL, a quorum quenching lactonase with unusual kinetic properties (2018), Sci. Rep., 8, 11262 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme bound to phosphare, glycerol or N-hexanoyl-L-homoserine lactone. The crystal structure of AaL reveals a unique hydrophobic patch which can relate to its high specificity, and highlights a flexible active site loop which may be involved in substrate binding Alicyclobacillus acidoterrestris
in complex with phosphate and glycol Alicyclobacillus acidoterrestris

Protein Variants

Protein Variants Comment Organism
V27G/P67Q/L72C/Y97S/Y99A/T177D/R223L/L226Q/L228M/W263H inactive Alicyclobacillus acidoterrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
0.0094
-
 N-butyryl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0174
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
0.0174
-
 3-oxo-dodecanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0185
-
 delta-decalactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0187
-
 delta-valerolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0369
-
 gamma-heptanolide in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0572
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
0.0572
-
 N-decanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.0827
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
0.0827
-
 N-hexanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
0.171
-
 gamma-butyrolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
0.171
-
 gamma-butyrolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
18.5
-
 delta-decalactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
18.7
-
 delta-valerolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
36.9
-
 gamma-heptanolide pH 8.3, 25°C Alicyclobacillus acidoterrestris

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.2 mM used in assay conditions Alicyclobacillus acidoterrestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an N-acyl-L-homoserine lactone + H2O Alicyclobacillus acidoterrestris
-
 an N-acyl-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidoterrestris T0BMH6
-
-
Alicyclobacillus acidoterrestris DSM 3922 T0BMH6
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep Trap column chromatography and Superdex 75 gel filtration Alicyclobacillus acidoterrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxo-dodecanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris 3-oxo-dodecanoyl-L-homoserine
-
 ?
an N-acyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris an N-acyl-L-homoserine
-
 ?
delta-decalactone + H2O
-
 Alicyclobacillus acidoterrestris 5-hydroxydecanoate
-
 ?
delta-decalactone + H2O
-
 Alicyclobacillus acidoterrestris 5-hydroxydecanoic acid
-
 ?
delta-valerolactone + H2O
-
 Alicyclobacillus acidoterrestris 5-hydroxypentanoic acid
-
 ?
delta-valerolactone + H2O
-
 Alicyclobacillus acidoterrestris DSM 3922 5-hydroxypentanoic acid
-
 ?
gamma-butyrolactone + H2O
-
 Alicyclobacillus acidoterrestris 4-hydroxybutanoic acid
-
 ?
gamma-butyrolactone + H2O
-
 Alicyclobacillus acidoterrestris DSM 3922 4-hydroxybutanoic acid
-
 ?
gamma-heptanolide + H2O
-
 Alicyclobacillus acidoterrestris 4-hydroxyheptanoic acid
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris DSM 3922 N-3-oxododecanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-butyryl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris DSM 3922 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris DSM 3922 N-hexanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AaL
-
 Alicyclobacillus acidoterrestris
N007_09425
-
 Alicyclobacillus acidoterrestris
quorum quenching lactonase
-
 Alicyclobacillus acidoterrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58.2
-
 Tm-value Alicyclobacillus acidoterrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
4.9
-
 3-oxo-dodecanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
5.2
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
5.2
-
 N-decanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
7
-
 gamma-heptanolide pH 8.3, 25°C Alicyclobacillus acidoterrestris
7
-
 gamma-heptanolide in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
8.4
-
 delta-valerolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
8.4
-
 delta-decalactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
8.4
-
 delta-valerolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
8.4
-
 delta-decalactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
12.6
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
12.6
-
 N-butyryl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
14
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
14
-
 N-hexanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
23.5
-
 gamma-butyrolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
23.5
-
 gamma-butyrolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris

General Information

General Information Comment Organism
physiological function the enzyme inhibits the biofilm formation of Acinetobacter baumannii Alicyclobacillus acidoterrestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
 gamma-heptanolide pH 8.3, 25°C Alicyclobacillus acidoterrestris
19
-
 gamma-heptanolide in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
92
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
92
-
 N-decanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
140
-
 gamma-butyrolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
140
-
 gamma-butyrolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
170
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
170
-
 N-hexanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
280
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
280
-
 3-oxo-dodecanoyl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
450
-
 delta-valerolactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
450
-
 delta-decalactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
450
-
 delta-valerolactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
450
-
 delta-decalactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris
1300
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
1300
-
 N-butyryl-L-homoserine lactone in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C Alicyclobacillus acidoterrestris