Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20-30% (w/v) PEG 8000 and 50 mM Tris-HCl buffer (pH 8.0) | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C258L/I261F/W263A | the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme | Saccharolobus solfataricus |
F46L/C258A/W263M/I280T | the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme | Saccharolobus solfataricus |
L72I/Y99F/I122L/L228M/F229S/W263L | the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme | Saccharolobus solfataricus |
V27A/I76T/Y97W/Y99F/L130P/L226V | the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme | Saccharolobus solfataricus |
V27A/Y97W/L228M/W263M | the mutant demonstrates a large increase in catalytic efficiencies over the wild-type enzyme, with increases of 2210fold, 163fold, 58fold, 16fold against methyl parathion, malathion, ethyl paraoxon, and methyl paraoxon, respectively | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fensulfothion | - |
Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an N-acyl-L-homoserine lactone + H2O | Saccharolobus solfataricus | - |
an N-acyl-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97VT7 | Sulfolobus solfataricus | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Superdex 75 gel filtration | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an N-acyl-L-homoserine lactone + H2O | - |
Saccharolobus solfataricus | an N-acyl-L-homoserine | - |
? | |
additional information | no activity with coumaphos, fensulfothion, fenitrothion, diazinon, chlorpyrifos, and ethyl parathion. Low activity with malathion, methyl parathion methyl paraoxon, and ethyl paraoxon | Saccharolobus solfataricus | ? | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Saccharolobus solfataricus | N-3-oxododecanoyl-L-homoserine | - |
? | |
undecanoic-delta-lactone + H2O | - |
Saccharolobus solfataricus | 5-hydroxyundecanoic acid | - |
? | |
undecanoic-gamma-lactone + H2O | - |
Saccharolobus solfataricus | 4-hydroxyundecanoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
POX | - |
Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.141 | - |
N-3-oxododecanoyl-L-homoserine lactone | mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
0.584 | - |
N-3-oxododecanoyl-L-homoserine lactone | mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
0.905 | - |
undecanoic-gamma-lactone | mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
1.63 | - |
undecanoic-gamma-lactone | mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
1.78 | - |
N-3-oxododecanoyl-L-homoserine lactone | mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
2.36 | - |
undecanoic-gamma-lactone | wild type enzyme, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
2.55 | - |
undecanoic-delta-lactone | wild type enzyme, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
2.55 | - |
undecanoic-delta-lactone | mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
5.33 | - |
undecanoic-delta-lactone | mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
7.5 | - |
undecanoic-delta-lactone | mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
16 | - |
undecanoic-gamma-lactone | mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
31.6 | - |
N-3-oxododecanoyl-L-homoserine lactone | wild type enzyme, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
360 | - |
undecanoic-delta-lactone | mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
440 | - |
undecanoic-gamma-lactone | mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
573 | - |
undecanoic-gamma-lactone | mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C | Saccharolobus solfataricus | |
9650 | - |
undecanoic-delta-lactone | mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C | Saccharolobus solfataricus |